Virus:Article Title: Exercise-induced activation of ventromedial hypothalamic steroidogenic factor-1 neurons mediates improvements in endurance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Homer1 Synaptic Systems Cat#160 003; RRID: AB_887730 Goat Anti-Rabbit 555 Invitrogen Cat#A-21428; RRID: AB_141784 Bacterial and virus strains AAV1-hSyn-FLEX-TeLCP2A-eYFP-WPRE Fan Wang (unpublished); NeuroTools (viral prep) Addgene plasmid #135391 AAV1-CAG-FLEX-eGFP Ian Wickersham (unpublished) Addgene AAV1; 59331-AAV1 AAV1-hSyn1-SIO-stGtACR2-FusionRed Mahn et al. 76 Addgene AAV1; 105677-AAV1 AAV1-hSyn-FLEX-GCaMP6s-WPRE-SV40 Chen et al. 77 Addgene AAV1; 100845-AAV1 AAV5-EF1a-double floxedhChR2(H134R)-eYFP-WPRE-HGHpA Karl Deisseroth (unpublished) Addgene AAV5; 20298-AAV5 AAV5-EF1a-DIO-eYFP Karl Deisseroth (unpublished) Addgene AAV5; 27056-AAV5 AAV1-CAG-DIO-mCherrymBDNF-shRNAmir Vector Biolabs shAAV-253926 Chemicals, Peptides, and Recombinant Proteins Isoflurane Piramal Cat#0010250P Zetamine (ketamine), 100 mg/mL Med-Vet Cat#RXKETAMINE Rumpun (xylazine), 100 mg/mL Med-Vet Cat#RXXYLAZINE-RUMP Loxicom (meloxicam), 5 mg/mL Norbrook Cat#55529-040-11 RNAlater Millipore Sigma Cat#R0901 Chloral hydrate Millipore Sigma Cat#C8383 Sodium chloride Fisher Chemical Cat#S271-3 Potassium chloride Millipore Sigma Cat#P9541 Magnesium chloride Millipore Sigma Cat#M4880 Calcium chloride Millipore Sigma Cat#C5670 Sodium phosphate monobasic Millipore Sigma Cat#S3139 Sodium bicarbonate Millipore Sigma Cat#S8875 Glucose Millipore Sigma Cat#G7528 Alexa Fluor 350 hydrazide ThermoFisher Cat#A10439 Potassium gluconate Millipore Sigma Cat#G4500 HEPES Millipore Sigma Cat#54457 EGTA Millipore Sigma Cat#E3889 Magnesium ATP Millipore Sigma Cat#A9187 Paraformaldehyde MP Biomedicals Cat#150146 Agarose Millipore Sigma Cat#A9539 Polyvinyl alcohol mounting medium with DABCO Millipore Sigma Cat#10981 Deposited data Data reported in the figures Open Science Framework https://osf.io/3879d/overview?view_ only=398cc5b1545f4b07ab29 ddf6ceba1fbc Experimental Models: Organisms/Strains Mouse: Tg(Nr5a1-Cre)7 Lowl /J The Jackson Laboratory RRID: IMSR_JAX:012462 Mouse: Ai9 B6.Cg-Gt(ROSA) 26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory RRID: IMSR_JAX:007909 (Continued on next page) Neuron 114, 1–12.e1–e9, May 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: SF1-eGFP Stallings et al. 78 ; Teppei Fujikawa, Keith L. Parker N/A Mouse: C57BL6/J The Jackson Laboratory RRID: IMSR_JAX:000664 Oligonucleotides Mm-Nr5a1 (SF1) RNAscope probe ACDbio Cat#445731-C2 Mm-Bdnf RNAscope probe ACDbio Cat#424821 Software and Algorithms Matlab R2024a MathWorks https://www.mathworks.com/ products/matlab RStudio 1.2.5019 Posit https://posit.co/download/ rstudio-desktop/ Bioconductor 3.8 Bioconductor https://bioconductor.org/ CellProfiler Stirling et al. 79 https://cellprofiler.org/ MetaScreen Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ MacroInterpreter Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ Synapse Tucker-Davis Technologies https://www.tdt.com/component/ synapse-software Prism 10 GraphPad https://www.graphpad.com Minian Dong et al. 80 https://github.com/denisecailab/minian CellReg Sheintuch et al. 42 https://github.com/zivlab/CellReg pCLAMP 11 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system/ acquisition-and-analysis-software/ pclamp-software-suite Easy Electrophysiology Easy Electrophysiology https://www.easyelectrophysiology.com Other Vanderbilt Mouse Metabolic Phenotyping Center Vanderbilt University RRID: SCR_021939 Purina 5001 Lab Diet N/A RNAscope manual assay ACDbio N/A TruSeq stranded mRNA kit Illumina Cat#20020595 TruSeq Unique dual indexes Illumina Cat#20022371 NextSeq 2000 Illumina N/A 4200 TapeStation Agilent Cat#G2991BA Qubit 4 Fluorometer Invitrogen Cat#Q33226 Dual Just for Mouse Stereotax Stoelting Cat#51733 Syringe pump for stereotaxic injections Harvard Apparatus Cat#703007 PHD Ultra Nanofil syringe World Precision Instruments Cat#NANOFIL-100 Metabond Parkell Cat#S380 Ortho-Jet Lang Dental Manufacturing Cat#1323 DEXA InAlyzer2 System Micro Photonics N/A Optic fiber for optogenetics Thorlabs Cat#FT200UMT Patch cable for optogenetics Thorlabs Cat#M72L02 Ferrule for optogenetics Kientec Systems Cat#FZI-LC-230 Optic fibers for photometry Doric Cat#MF2.5, 400/430-0.37 GRIN lens, 8mm, 600 μm diameter Inscopix Cat#1050-004600 (Continued on next page) e2 Neuron 114, 1–12.e1–e9, May 6, 2026
Plasmid Preparation:Article Title: Exercise-induced activation of ventromedial hypothalamic steroidogenic factor-1 neurons mediates improvements in endurance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Homer1 Synaptic Systems Cat#160 003; RRID: AB_887730 Goat Anti-Rabbit 555 Invitrogen Cat#A-21428; RRID: AB_141784 Bacterial and virus strains AAV1-hSyn-FLEX-TeLCP2A-eYFP-WPRE Fan Wang (unpublished); NeuroTools (viral prep) Addgene plasmid #135391 AAV1-CAG-FLEX-eGFP Ian Wickersham (unpublished) Addgene AAV1; 59331-AAV1 AAV1-hSyn1-SIO-stGtACR2-FusionRed Mahn et al. 76 Addgene AAV1; 105677-AAV1 AAV1-hSyn-FLEX-GCaMP6s-WPRE-SV40 Chen et al. 77 Addgene AAV1; 100845-AAV1 AAV5-EF1a-double floxedhChR2(H134R)-eYFP-WPRE-HGHpA Karl Deisseroth (unpublished) Addgene AAV5; 20298-AAV5 AAV5-EF1a-DIO-eYFP Karl Deisseroth (unpublished) Addgene AAV5; 27056-AAV5 AAV1-CAG-DIO-mCherrymBDNF-shRNAmir Vector Biolabs shAAV-253926 Chemicals, Peptides, and Recombinant Proteins Isoflurane Piramal Cat#0010250P Zetamine (ketamine), 100 mg/mL Med-Vet Cat#RXKETAMINE Rumpun (xylazine), 100 mg/mL Med-Vet Cat#RXXYLAZINE-RUMP Loxicom (meloxicam), 5 mg/mL Norbrook Cat#55529-040-11 RNAlater Millipore Sigma Cat#R0901 Chloral hydrate Millipore Sigma Cat#C8383 Sodium chloride Fisher Chemical Cat#S271-3 Potassium chloride Millipore Sigma Cat#P9541 Magnesium chloride Millipore Sigma Cat#M4880 Calcium chloride Millipore Sigma Cat#C5670 Sodium phosphate monobasic Millipore Sigma Cat#S3139 Sodium bicarbonate Millipore Sigma Cat#S8875 Glucose Millipore Sigma Cat#G7528 Alexa Fluor 350 hydrazide ThermoFisher Cat#A10439 Potassium gluconate Millipore Sigma Cat#G4500 HEPES Millipore Sigma Cat#54457 EGTA Millipore Sigma Cat#E3889 Magnesium ATP Millipore Sigma Cat#A9187 Paraformaldehyde MP Biomedicals Cat#150146 Agarose Millipore Sigma Cat#A9539 Polyvinyl alcohol mounting medium with DABCO Millipore Sigma Cat#10981 Deposited data Data reported in the figures Open Science Framework https://osf.io/3879d/overview?view_ only=398cc5b1545f4b07ab29 ddf6ceba1fbc Experimental Models: Organisms/Strains Mouse: Tg(Nr5a1-Cre)7 Lowl /J The Jackson Laboratory RRID: IMSR_JAX:012462 Mouse: Ai9 B6.Cg-Gt(ROSA) 26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory RRID: IMSR_JAX:007909 (Continued on next page) Neuron 114, 1–12.e1–e9, May 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: SF1-eGFP Stallings et al. 78 ; Teppei Fujikawa, Keith L. Parker N/A Mouse: C57BL6/J The Jackson Laboratory RRID: IMSR_JAX:000664 Oligonucleotides Mm-Nr5a1 (SF1) RNAscope probe ACDbio Cat#445731-C2 Mm-Bdnf RNAscope probe ACDbio Cat#424821 Software and Algorithms Matlab R2024a MathWorks https://www.mathworks.com/ products/matlab RStudio 1.2.5019 Posit https://posit.co/download/ rstudio-desktop/ Bioconductor 3.8 Bioconductor https://bioconductor.org/ CellProfiler Stirling et al. 79 https://cellprofiler.org/ MetaScreen Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ MacroInterpreter Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ Synapse Tucker-Davis Technologies https://www.tdt.com/component/ synapse-software Prism 10 GraphPad https://www.graphpad.com Minian Dong et al. 80 https://github.com/denisecailab/minian CellReg Sheintuch et al. 42 https://github.com/zivlab/CellReg pCLAMP 11 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system/ acquisition-and-analysis-software/ pclamp-software-suite Easy Electrophysiology Easy Electrophysiology https://www.easyelectrophysiology.com Other Vanderbilt Mouse Metabolic Phenotyping Center Vanderbilt University RRID: SCR_021939 Purina 5001 Lab Diet N/A RNAscope manual assay ACDbio N/A TruSeq stranded mRNA kit Illumina Cat#20020595 TruSeq Unique dual indexes Illumina Cat#20022371 NextSeq 2000 Illumina N/A 4200 TapeStation Agilent Cat#G2991BA Qubit 4 Fluorometer Invitrogen Cat#Q33226 Dual Just for Mouse Stereotax Stoelting Cat#51733 Syringe pump for stereotaxic injections Harvard Apparatus Cat#703007 PHD Ultra Nanofil syringe World Precision Instruments Cat#NANOFIL-100 Metabond Parkell Cat#S380 Ortho-Jet Lang Dental Manufacturing Cat#1323 DEXA InAlyzer2 System Micro Photonics N/A Optic fiber for optogenetics Thorlabs Cat#FT200UMT Patch cable for optogenetics Thorlabs Cat#M72L02 Ferrule for optogenetics Kientec Systems Cat#FZI-LC-230 Optic fibers for photometry Doric Cat#MF2.5, 400/430-0.37 GRIN lens, 8mm, 600 μm diameter Inscopix Cat#1050-004600 (Continued on next page) e2 Neuron 114, 1–12.e1–e9, May 6, 2026
Recombinant:Article Title: Exercise-induced activation of ventromedial hypothalamic steroidogenic factor-1 neurons mediates improvements in endurance.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Homer1 Synaptic Systems Cat#160 003; RRID: AB_887730 Goat Anti-Rabbit 555 Invitrogen Cat#A-21428; RRID: AB_141784 Bacterial and virus strains AAV1-hSyn-FLEX-TeLCP2A-eYFP-WPRE Fan Wang (unpublished); NeuroTools (viral prep) Addgene plasmid #135391 AAV1-CAG-FLEX-eGFP Ian Wickersham (unpublished) Addgene AAV1; 59331-AAV1 AAV1-hSyn1-SIO-stGtACR2-FusionRed Mahn et al. 76 Addgene AAV1; 105677-AAV1 AAV1-hSyn-FLEX-GCaMP6s-WPRE-SV40 Chen et al. 77 Addgene AAV1; 100845-AAV1 AAV5-EF1a-double floxedhChR2(H134R)-eYFP-WPRE-HGHpA Karl Deisseroth (unpublished) Addgene AAV5; 20298-AAV5 AAV5-EF1a-DIO-eYFP Karl Deisseroth (unpublished) Addgene AAV5; 27056-AAV5 AAV1-CAG-DIO-mCherrymBDNF-shRNAmir Vector Biolabs shAAV-253926 Chemicals, Peptides, and Recombinant Proteins Isoflurane Piramal Cat#0010250P Zetamine (ketamine), 100 mg/mL Med-Vet Cat#RXKETAMINE Rumpun (xylazine), 100 mg/mL Med-Vet Cat#RXXYLAZINE-RUMP Loxicom (meloxicam), 5 mg/mL Norbrook Cat#55529-040-11 RNAlater Millipore Sigma Cat#R0901 Chloral hydrate Millipore Sigma Cat#C8383 Sodium chloride Fisher Chemical Cat#S271-3 Potassium chloride Millipore Sigma Cat#P9541 Magnesium chloride Millipore Sigma Cat#M4880 Calcium chloride Millipore Sigma Cat#C5670 Sodium phosphate monobasic Millipore Sigma Cat#S3139 Sodium bicarbonate Millipore Sigma Cat#S8875 Glucose Millipore Sigma Cat#G7528 Alexa Fluor 350 hydrazide ThermoFisher Cat#A10439 Potassium gluconate Millipore Sigma Cat#G4500 HEPES Millipore Sigma Cat#54457 EGTA Millipore Sigma Cat#E3889 Magnesium ATP Millipore Sigma Cat#A9187 Paraformaldehyde MP Biomedicals Cat#150146 Agarose Millipore Sigma Cat#A9539 Polyvinyl alcohol mounting medium with DABCO Millipore Sigma Cat#10981 Deposited data Data reported in the figures Open Science Framework https://osf.io/3879d/overview?view_ only=398cc5b1545f4b07ab29 ddf6ceba1fbc Experimental Models: Organisms/Strains Mouse: Tg(Nr5a1-Cre)7 Lowl /J The Jackson Laboratory RRID: IMSR_JAX:012462 Mouse: Ai9 B6.Cg-Gt(ROSA) 26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory RRID: IMSR_JAX:007909 (Continued on next page) Neuron 114, 1–12.e1–e9, May 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: SF1-eGFP Stallings et al. 78 ; Teppei Fujikawa, Keith L. Parker N/A Mouse: C57BL6/J The Jackson Laboratory RRID: IMSR_JAX:000664 Oligonucleotides Mm-Nr5a1 (SF1) RNAscope probe ACDbio Cat#445731-C2 Mm-Bdnf RNAscope probe ACDbio Cat#424821 Software and Algorithms Matlab R2024a MathWorks https://www.mathworks.com/ products/matlab RStudio 1.2.5019 Posit https://posit.co/download/ rstudio-desktop/ Bioconductor 3.8 Bioconductor https://bioconductor.org/ CellProfiler Stirling et al. 79 https://cellprofiler.org/ MetaScreen Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ MacroInterpreter Sable Systems https://www.sablesys.com/products/ promethion-high-definition-roomcalorimetry-system/promethion-software/ Synapse Tucker-Davis Technologies https://www.tdt.com/component/ synapse-software Prism 10 GraphPad https://www.graphpad.com Minian Dong et al. 80 https://github.com/denisecailab/minian CellReg Sheintuch et al. 42 https://github.com/zivlab/CellReg pCLAMP 11 Molecular Devices https://www.moleculardevices.com/ products/axon-patch-clamp-system/ acquisition-and-analysis-software/ pclamp-software-suite Easy Electrophysiology Easy Electrophysiology https://www.easyelectrophysiology.com Other Vanderbilt Mouse Metabolic Phenotyping Center Vanderbilt University RRID: SCR_021939 Purina 5001 Lab Diet N/A RNAscope manual assay ACDbio N/A TruSeq stranded mRNA kit Illumina Cat#20020595 TruSeq Unique dual indexes Illumina Cat#20022371 NextSeq 2000 Illumina N/A 4200 TapeStation Agilent Cat#G2991BA Qubit 4 Fluorometer Invitrogen Cat#Q33226 Dual Just for Mouse Stereotax Stoelting Cat#51733 Syringe pump for stereotaxic injections Harvard Apparatus Cat#703007 PHD Ultra Nanofil syringe World Precision Instruments Cat#NANOFIL-100 Metabond Parkell Cat#S380 Ortho-Jet Lang Dental Manufacturing Cat#1323 DEXA InAlyzer2 System Micro Photonics N/A Optic fiber for optogenetics Thorlabs Cat#FT200UMT Patch cable for optogenetics Thorlabs Cat#M72L02 Ferrule for optogenetics Kientec Systems Cat#FZI-LC-230 Optic fibers for photometry Doric Cat#MF2.5, 400/430-0.37 GRIN lens, 8mm, 600 μm diameter Inscopix Cat#1050-004600 (Continued on next page) e2 Neuron 114, 1–12.e1–e9, May 6, 2026
|
Addgene inc is a verified supplier 